Histo Pot 6

Histology

SUBMIT HISTOLOGY SAMPLES  


Collecting Histopathological Samples 

Optimal sampling for histopathological investigation will depend on the clinical situation. For standard assessment of a group of farmed fish, we generally recommend sampling from a minimum of 5 individuals, collecting the following tissues: 

  • gills
  • heart
  • pyloric caecae (includes pancreas in salmonids)
  • liver
  • spleen
  • hindgut
  • kidney 
  • skin/muscle over the lateral line


Tissues from each individual should be placed in separate leak-proof containers with 10% buffered formalin. In general, the volume of formalin should be at least 10 times the total volume of tissue.

  • Our vets are always happy to advise on sample collection and handling, and can advise on diagnostic sampling strategies appropriate for your farm or facility
  • We can provide clients with instructional videos for histopathological sampling of the following species on request: Atlantic salmon (including fry), rainbow trout, lumpsucker, ballan wrasse, zebrafish and tilapia
  • Full histopathological sampling equipment (including formalin- lled containers and instrumentation) can be provided on request
Histo Pot 7

Sending Histopathological Samples To Fish Vet Group 

For the best possible quality of analysis Fish Vet Group recommend: 

  • Samples should be shipped as soon as possible after sampling
  • Samples should be kept cool — but not frozen — until shipment. Freezing hampers histopathological examination 
  • Avoid spills on the packaging/ submission form 
  • Please label all samples clearly and unambiguously. The labeling should also be indicated on the requisition form

All samples should be packed in three layers of packaging: 

  • One or more leak-proof primary receptacles. Use an airtight container large enough for the tissue pieces to move freely in the fixative 
  • A leak-proof secondary container (especially important for liquid samples) 
  • A sufficiently strong outer packaging (padded envelope, stiff cardboard, polystyrene etc.) 
  • For liquid samples please include plenty of absorbent material between the primary and secondary packaging 
  • Also see Post Office regulations for the shipment of biological materials 


Please label the package: “BIOLOGICAL SUBSTANCE CATEGORY B” 

All samples should be sent to: 

Fish Vet Group

22 Carsegate Road, 

Inverness, Scotland, 

IV3 8EX 

 

Samples from Inside the European Union

Formalin- fixed fish tissues may be freely sent into the UK from the EU, provided packaging meets local regulations. We recommend that aquaculture producers outside the UK wishing to submit samples notify, or preferably make contact through, their prescribing veterinary surgeon before sending samples to us.

Samples from Outside the European Union

We are happy to accept samples from outside the EU, though an import licence must be granted from the UK’s Animal and Plant Health Authority (APHA) before samples may be brought into the UK (in our experience this may take up to two weeks when importing for the first time). For detailed advice on this please contact us. 

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