Pcr Tubes 2

PCR

SUBMIT PCR SAMPLES 

Collecting PCR Samples

For the best possible quality of analysis we recommend having the following equipment available:

  • Labelled tubes containing RNAlater or an FVG tube rack, which can be provided on request
  • Cooling element (4°C) or ice 
  • Suitable packaging for sending samples
  • Sterile tweezers and scalpel
  • Gas burner for sterilization of tweezers and a scalpel handle
  • Sterile surface for trimming tissue samples (a microscope slide or the inside of the scalpel blade packaging is suitable) 
  • Paper for removing tissue remains on the scalpel blade 
  • 70% alcohol and/or 10% bleach solution for disinfection of sampling equipment 


Sampling Procedure

  • Tissue samples should be about 2x2x2mm (the size of a match head) to ensure good preservation. Avoid using the abdominal cavity when trimming tissues
  • Preferably sample two pieces of tissue per organ, add both pieces to the same tube (A and B sample)
  • Ensure that the tissue samples are covered with RNAlater to ensure proper conservation
  • Place the tube with the tissue samples on a cooling element or on ice immediately after sampling
  • Sterilize the equipment between each fish to avoid cross contamination
  • We recommend that experienced personnel perform the sampling
Pcr Tubes 3

Storage 

Store tissue samples in RNAlater at 4°C (refrigerator) after sampling.

If the samples are not to be sent within the first 48 hours after sampling, store the samples at 4°C for 24 hours to complete the conservation process, then transfer the samples to a freezer (-20°C).

Sending PCR Samples to Fish Vet Group

Samples should be sent in a Styrofoam box or bubble envelope with a cooler/ice pack. Please send samples as quickly as possible after sampling, accompanied with the name and address of the sender and notes with relevant clinical details. 

Salmon PCR video
main.css loaded.