SUBMIT PCR SAMPLES
Collecting PCR Samples
For the best possible quality of analysis we recommend having the following equipment available:
- Labelled tubes containing RNAlater or an FVG tube rack, which can be provided on request
- Cooling element (4°C) or ice
- Suitable packaging for sending samples
- Sterile tweezers and scalpel
- Gas burner for sterilization of tweezers and a scalpel handle
- Sterile surface for trimming tissue samples (a microscope slide or the inside of the scalpel blade packaging is suitable)
- Paper for removing tissue remains on the scalpel blade
- 70% alcohol and/or 10% bleach solution for disinfection of sampling equipment
- Tissue samples should be about 2x2x2mm (the size of a match head) to ensure good preservation. Avoid using the abdominal cavity when trimming tissues
- Preferably sample two pieces of tissue per organ, add both pieces to the same tube (A and B sample)
- Ensure that the tissue samples are covered with RNAlater to ensure proper conservation
- Place the tube with the tissue samples on a cooling element or on ice immediately after sampling
- Sterilize the equipment between each fish to avoid cross contamination
- We recommend that experienced personnel perform the sampling
Store tissue samples in RNAlater at 4°C (refrigerator) after sampling.
If the samples are not to be sent within the first 48 hours after sampling, store the samples at 4°C for 24 hours to complete the conservation process, then transfer the samples to a freezer (-20°C).
Sending PCR Samples to Fish Vet Group
Samples should be sent in a Styrofoam box or bubble envelope with a cooler/ice pack. Please send samples as quickly as possible after sampling, accompanied with the name and address of the sender and notes with relevant clinical details.