Pcr Tubes 2

PCR

SUBMIT PCR SAMPLES 

Collecting PCR Samples

For the best possible quality of analysis we recommend having the following equipment available:

  • Labelled tubes containing RNAlater or an FVG tube rack, which can be provided on request
  • Cooling element (4°C) or ice 
  • Suitable packaging for sending samples
  • Sterile tweezers and scalpel
  • Gas burner for sterilization of tweezers and a scalpel handle
  • Sterile surface for trimming tissue samples (a microscope slide or the inside of the scalpel blade packaging is suitable) 
  • Paper for removing tissue remains on the scalpel blade 
  • 70% alcohol and/or 10% bleach solution for disinfection of sampling equipment 


Sampling Procedure

  • Tissue samples should be about 2x2x2mm (the size of a match head) to ensure good preservation. Avoid using the abdominal cavity when trimming tissues
  • Preferably sample two pieces of tissue per organ, add both pieces to the same tube (A and B sample)
  • Ensure that the tissue samples are covered with RNAlater to ensure proper conservation
  • Place the tube with the tissue samples on a cooling element or on ice immediately after sampling
  • Sterilize the equipment between each fish to avoid cross contamination
  • We recommend that experienced personnel perform the sampling
Pcr Tubes 3

Storage 

Store tissue samples in RNAlater at 4°C (refrigerator) after sampling.

If the samples are not to be sent within the first 48 hours after sampling, store the samples at 4°C for 24 hours to complete the conservation process, then transfer the samples to a freezer (-20°C).

Sending PCR Samples to Fish Vet Group

Samples should be sent in a Styrofoam box or bubble envelope with a cooler/ice pack. Please send samples as quickly as possible after sampling, accompanied with the name and address of the sender and notes with relevant clinical details. 

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